Taking chrysanthemum as the test material, 120 thick and uniform chrysanthemum stems were selected and divided into two groups, 60 in each group. The leaves were cut about 12cm long, and the base was cut into wedge-shaped surfaces. The basal part was treated with naphthalene hexanoate for 12 hours, then propagating in the intelligent seedbed of natural light and the red seedbed with the plant growth lamp supplementing light, and the growth of the stem segment was observed and recorded, respectively. 10PPM The extraction method is used for the determination of chlorophyll content. On the 3rd \ 6th \ 12th day of culture, evenly take 0.2g of leaves from the same parts of each treatment, cut them into pieces, soak them in 1:1 acetone: anhydrous ethanol, extract them in a 40 ℃ incubator for 24 hours, measure the OD value at the wavelength of 652nm, and calculate the chlorophyll content. The soluble sugar was determined by 3,5 dinitrosalicylic acid method, and the activity of nitrate reductase (NR) was determined by sulfanilamide colorimetry
1. After 30 days of culture, the rooting of stem segments under red light is earlier than that under natural light, and the number of final roots is more, the rooting rate is as high as 100%, and the roots are more and stronger. The leaves are thick green, the stems are strong and the seedlings are vigorous. In the whole culture process, the growth of the material under red light was obviously better than that under natural light, which showed that red light had the effect of promoting the growth of root.
2. In the process of stem growth, the content of chlorophyll first decreased and then increased under natural light or red light. However, the content of chlorophyll in red light was higher than that in natural light, which indicated that red light promoted the formation of chlorophyll obviously, and the result was more obvious with the increase of cultivation days. Under the red light, the growth of the plant is better, which may be due to the higher chlorophyll content in the plant, the more vigorous photosynthesis, and the more carbohydrate synthesis, thus providing sufficient material and energy for the growth of the plant.
3. The content of soluble sugar on the 9th day was lower than that on the 15th day, and it decreased more under red light than that under natural light. The rooting of stem segments under red light was earlier than that under natural light. After 15 days, the content of soluble sugar in red light is higher than that in natural light, which may be related to the higher chlorophyll content in red light (Table 2) and the more vigorous photosynthesis.
4. The NR activity of stem segment under red light was significantly higher than that under natural light (Table 2). It can be seen that red light can promote nitrogen metabolism of chrysanthemum stem.
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